Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates.

نویسندگان

  • M A Pfaller
  • Q Vu
  • M Lancaster
  • A Espinel-Ingroff
  • A Fothergill
  • C Grant
  • M R McGinnis
  • L Pasarell
  • M G Rinaldi
  • L Steele-Moore
چکیده

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 32 7  شماره 

صفحات  -

تاریخ انتشار 1994